Journal de microbiologie alimentaire et industrielle

RT-qPCR (ISC-RT-qPCR) Method for Detection of Human Norovirus in Food and Environmental Sample.

Abstract

Pen Tan

Human noroviruses (HuNoVs) are the main cause of acute non- bacterial gastroenteritis worldwide. In this analysis, a novel in situ mediated viral receptor RT-qPCR (ISC-RTqPCR) was used to detect HuNoVs in oysters and compared to the standard RT-qPCR system. Ten HuNoVs RT-PCR positive and 5 negative clinical samples from patients with gastroenteritis were used to equate the specificity and sensitivity of the ISC- RT-qPCR with that of the RT-qPCR assay. ISC-RT-qPCR had a one-log and two-log increase in sensitivity relative to the RT- qPCR studies for genotype I (GI) and GII, respectively. HuNoV concentrations in oyster tissues have been studied in artificially inoculated oysters.