Journal du syndrome métabolique

Biomarkers of Oxidative Stress in Syndrome Metabolic Patients, a Case Control Study

Abstract

Francisco Avilés-Plaza , Juana Bernabé , Begoña Cerdá , Javier Marhuenda, Pilar Zafrilla , Tânia O. Constantino , Juana Mulero, Cristina García-Viguera , Diego A. Moreno, José Abellán and Soledad Parra-Pallarés

Background: Owing to the proposal of the increase of oxidative stress (OxS) as an early event in the development of the metabolic syndrome (MetS), the aim of the present study was to evaluate certain OxS biomarkers in patients with MetS compared to healthy people age-matched and younger to assess the relevance of aging in OxS and MetS.

Methods: A total of 72 patients, 32 who fulfilled the Adult Treatment Panel III criteria for the MetS and 40 individuals without MetS, 20 age-matched to the MetS patients (Control I) and 20 younger subjects (Control II) were studied. We measured several anthropometric and serum parameters and two kinds of molecules related to OxS: modified molecules by reactive oxygen species (ROS) such as oxidized LDL (oxLDLc), and consumed or inducted molecules (enzymes or antioxidants such as Glutathione reductase GR,) associated with ROS metabolism. The statistical analysis was performed using SPSS v18.0.

Results: Only significant differences were observed in the values of GR between the MetS patients and Control I (50.31 ± 8.15 U/L vs 59.50 ± 9.98 U/L). We found significantly higher levels in the MetS patients compared to Control II of oxLDLc (96.77 ± 23.05 U/L vs 60.17 ± 16.28 U/L), F2 -isoprostanes (3.17 ± 1.78 µg/g creatinine vs 2.04 ± 0.80 µg/g creatinine) and protein cabonils (PC) (0.56 ± 0.26 nmol/mg vs 0.29 ± 0.13 nmol/mg).

Conclusions: Results have shown that MetS patients don’t present a superior OxS in comparison to age-related healthy individuals. Finally, aging is more relevant to OxS than MetS per se.