Journal des bioprocédés et des biotechniques

Separation, Purification and Chemical Structure Examination of Antifungal Compound from Streptomyces herbaricolor Biomcc-A.RP-131

Abstract

Ahmad Marasabessy, Rudiyono Rudiyono and Diana Dewi

An actinobacteria of Indonesia origin identified as Streptomyces herbaricolorBioMCC-a.RP-131 has potential antifungal activities. In our earlier study, this strain exhibited a broad spectrum of in vitro antifungal activity against various type strains of fungus (molds and yeasts). Fermentation of this strain in a complex medium lasted for 8 days to produce a broth having high antifungal activity for separation. Extraction of 2 litre fermentation broth with n-Butanol (1:1) produced 24 grams of dried crude extract. Separation of active compound via two times fractionations of the crude extract in silica gel column chromatography eluted with co-solvent in gradient was able to separate almost all impurities. From the analysis of UHPLC-MS spectrum, we found the final fraction still contained 2-4 components with 12 possible chemical formulas. One of the formula was C39H39N4 (MW 562.3175 g.mol-1) not reported yet in Chemspider database, suggesting it to be a new compound. Further work of separation is required to obtain single antifungal compound for chemical structure elucidation with UHPLC-MS and NMR.