Médecine moléculaire et génétique

Molecular Analysis of Erwinia Species Based on 16S rDNA Gene Sequences

Abstract

Ahmad I Funjan*

Traditional identification methods for the soft rot Erwinias are both imprecise and time-consuming. We have used the 16S rDNA to aid in their identification.
Analysis of 16S rDNA-PCR and 16S rDNA-RFLP and gene sequencing was found to be simple, precise, and rapid method compared to other molecular techniques.
Analysis of the isolates genome by using their total DNA by amplifying their genome using the universal primer (fD1/rP2) indicated an amplified product of the
16S rDNA at 450 bp and the amplification using the specific 16S rDNA (EP16A/EP1GTC) was located of 700 bp. The restriction analysis of the universal amplified
product of 450 bp size using Hind III, proved the presence of different RFLP bands with some common bands. Variation in RFLP profile bands is an indication of
polymorphism in between the isolates. Whereas common bands indicates genetic stability among the isolates of the same spices or same genus. Sequencing of
the 16S rDNA universal primer amplified product showed a complete sequence of one isolate out of 10 isolate and the similarity between this isolate and the data
base indicated the presence of high similarity above 95% with other Enterobaceriaceae.