Journal de pharmacognosie et de produits naturels

Inter-Specific Variation Studies among Nephrolepis using SDS-PAGE

Abstract

Johnson M

The present study was aimed to reveal the biochemical similarity and varaition among the three taxonomically confused species Nephrolepis exaltata (L.) Schott, Nephrolepis auriculata (L.) Trimen and Nephrolepis multiflora (Roxb.) Jarret using SDS-PAGE analysis. To reveal the inter-speciifc variation among the selected Nephrolepis, SDS-PAGE was carried out to obtain protein bands following the method described by Anbalagan. Multiple regions (8) of activity were observed from protein  electrophoretic system of Nephrolepis. A total of 19 bands with various Rf values and molecular weight were demonstrated in the SDS-PAGE gel system of Nephrolepis. Among the three species of  Nephrolepis, N. exaltata showed maximum number of protein bands (9) followed by N. multiflora (6) and N. auriculata (4). Each region expressed different proteins which act as representative of the expression of a particular gene in the studied species of Nephrolepis. The similarity indices were calculated and cladogram was constructed based on the protein profiles of Nephrolepis and revealed the similarities and variation among the studied Nephrolepis species. The results obtained in this work also showed that SDS-PAGE analysis can provide an easy, low cost and quick way for the identification of inter-specific variation among the selected Nephrolepis species. SDS-PAGE analysis provided strong basis for the discrimination of genotypes on the basis of specific polypeptide fragments.