Microbiologie médicale et diagnostic

In Vitro Evaluation of Antimicrobial Effect of Extracts of Medicinal Plant’s Leaves

Abstract

Ale Zehra, Syed Baqir Shyum Naqvi and Syeda Qadam Ali

Abstract

In ancient and modern epoch, aerial parts of herbal plants have been broadly used for the treatment of primary health care and variety of ailment across the world depends on geographical cultivation. Now a day’s researcher focuses their attention to explore the plants having broad spectrum therapeutic activity. On the basis of medicinal plant activity surveillance, the core goal step of current study is to evaluate the susceptible antimicrobial activity of crude 50% hydro alcoholic extract of leaves of different folk medicinal plant i.e Ocimum basilicum L., Cymbopogon citratus (DC.) Stapf., Olea europaea L., Eucalyptus camaldulensis Dehnh. against different clinical isolates (Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcu epidermidis, Salmonella typhi and Candida albican) of microbial disease so as to trip up on the other alternatives and overcome the upcoming era of increasing microbial resistance. These isolates were collected from different hospitals and pathological laboratories of Karachi, Pakistan. Extract were obtain by soaking the leaves in 50% methanol and then vacuum dried through Rotavapor while the antimicrobial activity were evaluated by well diffusion method. Overall outcome of current study endorse that among selected herbs, Olea europea possessed broad spectrum antimicrobial activity. Further investigation is needed to develop formulation from the same plant. It’s timely need to explore the antimicrobial activity of other herbs also.