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Ethanol/Chloroform Precipitation-based PCR Amplifiable Method for DNA Extraction from the Human Skeletal Remains Recovered from Gandhara Grave Sites in Pakistan

Abstract

Basit A and Hilal M

The study of DNA extraction from bones is an important part of archaeogenetics. This study has provided a new investigating power to view and understand the human past. Gandhara Graves are widespread throughout the Gandhara region, from Bajaur to the Indus in Pakistan. The graves are not only widespread throughout Gandhara but other regions (the areas east-of the Indus River) as well. Dani has divided Gandhara Graves into three distinct groups belong to three distinct periods. In the present article, the systematic research has been undertaking by the researchers to isolate DNA from the fragments of human skeletal remains recovered from Gandhara graves. Although in this regards the researchers have mentioned novel methods that could be used for DNA extraction but the only method applied is ethanol/chloroform precipitation-based PCR amplifiable method for DNA Extraction from the human skeletal remains recovered from Gandhara grave sites.

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