Médecine moléculaire et génétique

Discrimination of Stem Cell Fate via Targeting of BAX, BAK1 and BOK Genes by Hsa-Mir-765

Abstract

Dokaneheifard S, Soltani BM, Soltaninejad H, Ghiasi P, Hosseinkhani S and Baharvand H

Apoptosis is a way for loss of unwanted cells during homeostasis, development, and integrity of tissues which is a process depending on caspases started through extrinsic and intrinsic or mitochondrial pathways. During a bioinformatics survey, important targeting miRs in both pathways have been identified. In the current study, due to common biochemical origin of apoptosis and differentiation, the role of hsa-miR-765 during the course of apoptosis induction and cardiomyocytes differentiation of hESCs have been investigated. We found the expression of BAX, BAK1, and BOK as the key elements of mitochondrial membrane pores involved in apoptosis induction and targeted by this miRNA during apoptosis and differentiation. All data represented here may disclose the imperative function of hsa-miR-765 in discrimination of biochemical aspect of differentiation and apoptosis of embryonic stem cells towards cardiomyocytes.