Journal des nanosciences : recherche actuelle

Development of a Sba-15 Mesoporous Silica Matrix and Functionalizade with Organic Groups to Purification of the Ribonucleic Acid

Abstract

Diaz Cano Cesar Alejandro, Nava Mendoza Rufino and Campos Guillen Juan

Ribonucleic acid (RNA) is of great importance because it ranges from laboratory tests, to diagnoses of viral, bacterial and parasitic diseases, inherited disorders and tumors, and even basic research. To provide reliable results, molecular biology techniques are used to extract it from prokaryotic or eukaryotic cells, but it is necessary to obtain pure and intact RNA biomolecules. Therefore, purifying the RNA is a critical step to obtain good quality RNA molecules (pure and intact). For these reasons, this thesis project will develop mesoporous silicas of the SBA-15 type functionalized with amino and mercaptan, as adsorbent materials for RNA purification. These functionalized mesoporous silicas that must have adequate textural properties (pore diameter in the mesoporous range of 7 nm and high surface area of up to 950 m2 / g) will allow the adsorption of RNA biomolecules on their surface through functional groups. Furthermore, these adsorbent materials are expected to purify RNA molecules with uniform size. In order to find an explanation for the behavior of adsorbent materials in RNA purification, they will be characterized by low angle X-ray Diffraction (SAXRD, Small-Angle X-Ray Diffraction), Raman spectroscopy, thermal analysis and gel electrophoresis.