Génétique humaine et Embryologie

Characteristic of the Diacylglycerol Acyltransferase 1 (DGAT1) K232A Gene in Sudanese Dairy Cattle (Kenana and Butana)

Abstract

Safa A. Mohammed Ali, M. K. A. Ahmed, M. Reissmann and G. A. Brockmann

The objective of the study was the characterization of DGAT1 variant in Sudanese dairy cattle breeds. In this study, we examined 94 Kenana and 91 Butana dairy cattle from two regions of Sudan. We genotyped the DGAT1 sequence variant AJ318490.1:g.10433/10434 AA>GC that leads to the Lysine-Alanine substitution at position 232 (K232A) in the protein. Genotyping was performed by allele specific PCR (KASP assay methods). In both breeds, the DGAT1 Lysine variant (232KA) that is associated with high fat and protein content as well as high fat yield in other breeds is the high frequent allele. The frequencies of the 232K allele were 96.3% and 84.6% in Kenana and Butana breeds, respectively. In conclusion, the two examined Sudanese dairy cattle breeds do not differ in allele frequencies at the DGAT1 loci.