Médecine moléculaire et génétique

Association by Polymorphism of the IL22 Gene in Situations, rs867810424 (A/G) and rs1390124543 (A/G) with a Risk of Infertility in Women

Abstract

Farideh Orooji, Sirous Naeimi and Mohammad Mehdi Moghani Bashi

Infertility is a growing and effective social disease in family relationships. The importance of controlling and measuring patient risk is determined by the individual's predisposing factors to infertility, genetic and acquired background. Inflammatory disorders are seen in many diseases, and cytokines, including IL22, play a role. IL22 is a precursor cytokine and has a dual role in are associated with increased egg fertility and the expansion of fatal growth. The aim of this study was to investigate the effect of two variants rs86781042 and rs1390124543 in IL22 on female infertility in southern Iran and compare it with healthy individuals. In this case study, evidence was used to study the polymorphism of the IL22 gene in the blood of 200 infertile and healthy patients in Shiraz hospitals, for DNA extraction and purification, using Salting Out and Proteinase K methods, followed by electrophoresis and PCR ARM was used. The results were analysed using SPSS software and Hardy Weinberg equilibrium. According to the results, it seems that the expression of two polymorphisms of IL22 gene is directly related to infertility in women in southern Iran and by using this relationship; it can be used as a biomarker for screening infertile women and diagnosing the disease.