Elrofaei NA, Elsharif KH, Elshikh AA, Bashir ME, Ahmed IF, Garbi MI, Kabbashi AS and Saleh MS
The petroleum ether, methanol and chloroform extracts of five plants were evaluated to detect antibacterial activity against five standards bacterial strain viz Bacillus subtitles (NCTC 8236), Klebsiella pneumonia (ATCC 53657), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923), using well-diffusion agar diffusion method. The petroleum ether and chloroform extracts were inactive compared to methanol extracts. The maximum antibacterial activity against the test organisms was found in methanol extract. Methanol extract of Citrullus colocynthis had maximum inhibitory activity (32 mm) against Escherichia coli. The MIC (minimum inhibitory concentration) of extracts was observed using well diffusion method. Amongst Gram negative bacteria Escherichia coli being inhibited at <3.12 mg/ml by Citrullus colocynthis root methanolic extract.
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