Biologie moléculaire : accès libre

Recent developments in Halotag technology

Abstract

Abdul Razzaq, Kanza Batool, Mahnoor Aslam, Aniqa Bhatti

Several biological functions and eukaryotic proteomes are interrelated and they depend upon protein function and analysis.Protein function and interaction is a biological process.. Deviations in these interactions can occur. In order to overcome this problem, scientists have devised a fusion platform called HaloTag technology. It is a technique used for functional analysis of proteins. This technique involves development of a 33kDa HaloTag that is fused to protein of interest and development of HaloTag ligand.When the HaloTag fusion protein tag and ligand come in contact, a covalent bond is formed rapidly. The covalent bond formed is irreversible so it ensures efficient isolation and purification of target protein. Variety of ligands is available for analysis of protein.Proteins always react differently to the new therapeutic products and it is unpredictable to discover their role after interacting with the ligand. HaloTag technology is used for analysis of protein-protein interactions, protein DNA interactions, protein functional analysis, enzyme immobilization and tumor cell detection. HaloTag technology evaluated the function in a way that it captures only protein of interest that is captured and purified.