Journal de bioanalyse et de biomédecine

Pharmacokinetics of Diminazene Aceturate in Buffalo Calves

Abstract

Hemant Kumar Pandey, Kaushal Kumar Singh, Birendra Kumar Roy and Suruchi Kumari

The pharmacokinetic study of diminazene aceturate (DMZ) was carried out in two separate groups of 4 e ach clinically healthy female Murrah buffalo calves aft er single dose i.v. (8 mg/kg) and i.m. (16 mg/kg) administrat ion. The mean free peak serum concentration of DMZ (CS max ) after i.v. (26.28±0.67μ g/ml) and i.m. (8.41±2.43 μ g/ml) a dmin- istrations were obtained at t max of 5 and 30 min respectively. The DMZ serum concentrations time data were best fi tted to the two compartment open model. The calculated s erum half life (t ½ β ) values of DMZ were 15.099±2.504 and 14.225±2.682 h after i.v. and i.m. administration r espec- tively. The mean values of total body clearance rat e of DMZ (ClB) after i.m. (3.785±1.119 ml/kg/min) was signif icantly higher (P<0.05) as compared with the i.v. (0.537±0. 063 ml/kg/min). DMZ was highly bound (77.14 to 94.40%) to buffalo calf plasma protein and its penetration int o erythro- cytes increased with increasing concentrations in b lood (7.60 to 33.00μ g/ml). Based on pharmacokinetic profiles, the satisfactory dosage regimens of diminazene aceturat e in buffalo calves were derived (2 mg/kg, i.v. and 12mg /kg, i.m). In case of emergent disease conditions to ens ure high DMZ serum concentrations, i.v. route may be preferr ed over i.m. route.