Journal de cytologie et d'histologie

Optimization and Validation of a Fingerprint about Hypericum perforatum L.Extracts by Plackett-Burman Randomization Method

Abstract

Shu Jie Shen, Yan Ru Liu, Chun Hua Guo, Rong Qing Huang, Jian Yun Yang and Bing Kun Xiao

Hypericum perforatum L. (HPL) has been used as a beneficial herb on menopause related syndromes for many years by inhibiting lipid oxidation. Hypericum perforatum L. extract contain flavonoids and phenolic acids, but it was not a method to separate and identify flavonoids which was based on design of experiment. Moreover, upper complicated HPLC method might cause undesirable effects on feasibility and repeatability in fingerprint technology. It was necessity to establish a generally and systematized way which could develop a reliable fingerprint technology for Chinese medicine. Plackett-Burman design method was a conventional tool for variables randomization aiming at optimization. Our study was based on this optimized design of experiment about ethanol extract from Hypericum perforatum L. and established the corresponding method for separation and identification of flavonoids. With this Design-Expert software, the results showed HCRF have significant effect on variables. From One-factor plots for main effect of HCRF, 254 nm wavelength measurement have a significant influenced on the HCRF, and have provided the optimized parameters.