Journal d'informatique et de biologie des systèmes

Homology Modelling of Hypoxanthin–Guanine Phosphoribosyltransferase, Enzyme Involved in Salvage Pathway of Purine Metabolism

Abstract

Jyotsna Choubey, Ashish Patel, Sheetal Khatri, Ruby Dewangan, Shailendra Kumar Gupta and M.K. Verma

Hypoxanthin-guanine phosphoribosyltransferase (HPRT) is responsible for catalyzing a reaction which breaks down uric acid in the body. The lack of this enzyme creates a build-up of uric-acid, leading to a neurological problem, joint pain and kidney problem. The condition is called Lesch-Nyhan Syndrome. A comparative modelling of the protein Hypoxanthin-guanine phosphoribosyltransferase is performed using the modeling program modeller9v3.The following structure validation programs PROCHECK, PROSA, VERIFY3D and WHATIF are used. Descret Optimized Protein Energy (DOPE), is a statistical potential used to asses homology model in protein structure prediction. Predicted model can be useful to develop new inhibitor against Lesch-Nyhan Syndrome. CASTp can be used to study surface features and functional regions of proteins.