Journal de bioanalyse et de biomédecine

Effect of Magnesium Sulfate on Mitochondrial Oxygen Consumption Rate In vitro Study

Abstract

Amna Al Zaabi and Aiman Al-Rahmani*

Hypoxic insults initiate a cascade of biochemical events that result in irreversible neuronal damage. Magnesium sulfate agent has a possible neuroprotective effect as it can work at different stages of hypoxic brain injury.
Objectives: Magnesium sulfate is used in the neonatal management of HIE as an adjunct medication to reduce Hypoxic Neuronal injury. In this Vitro study, we aimed to examine the efficacy of using magnesium sulfate in human cells to reduce oxygen consumption. We used in-vitro method utilizing foreskin as human cell surrogate.
Method: Foreskin specimens from healthy newborns were collected immediately after circumcision and processed within 1 h for measuring the cellular rate of O2 consumption. Samples were placed in the oxygen phosphorescence analyzer and allowed to run for approximately 1 h. Injection then added magnesium sulfate at different doses into the vial. We analyze the O2 consumption in the samples at different levels of Magnesium Sulfate; the results were plotted using Kaleida Graph TM software.
Results: The rate of respiration reduced with increasing the dose of MgSo4. The Cumulative analysis of cellular respiration rate was before and after an addition of MgSO4. Collectively yielding a (k) value of 0.08 μM O2/min μM O2 min-1 mg- and 0.04 M O2/min μM O2 min-1 mg- respectively with a significant P-value of <0.001.
Conclusion: Magnesium sulfate reduces the rate of O2 Consumption in a dose-dependent manner.