Journal de bioanalyse et de biomédecine

Development of a Rapid Point-of-Care Diagnostic Test for Zika Virus Infection Based on CRISPR-Cas12a Technology

Abstract

Coralie Reimerink

The outbreak of Zika virus has posed significant global health challenges, necessitating the development of rapid and accurate diagnostic tools. This study presents the development of a point-of-care diagnostic test for Zika virus infection utilizing CRISPR-Cas12a technology. Leveraging the specific and robust targeting capability of CRISPR-Cas12a, we designed a diagnostic platform capable of detecting ZIKV with high sensitivity and specificity. The assay demonstrated a limit of detection comparable to existing laboratory-based methods but with the advantage of faster results and minimal equipment requirements. This CRISPR-Cas12a-based diagnostic test offers a promising solution for timely and effective ZIKV detection, especially in resource-limited settings.