Journal d'informatique et de biologie des systèmes

A Novel Algorithm to Design an Efficient siRNA by Combining the Pre Proposed Rules of siRNA Designing

Abstract

Shishir Kumar Gupta, Bashir Akhlaq Akhoon, Mugdha Srivastava and Shailendra Kumar Gupta

Short interfering RNAs (siRNAs) can be used to suppress gene expression and have a lot of potential applications in therapy, yet how to design an effective siRNA is still under consideration. Numerous siRNA design tools have been developed recently. The set of candidates reported by these tools is usually large and often contains ineffective siRNAs. We initiated with the filtering of ineffective siRNAs, specifically, the current work establishes relationship between mentioned theoretically calculated property of subsequences and their efficiency for RNAi for any input set of experimental data. We combined some reported algorithms and designed a new algorithm that mainly focuses on stability and off-targeting to design siRNA sequences.