Journal d'immunobiologie

The process of healing a wound involves an extremely well-ordered series of actions, including hemostasis, inflammatory cell infiltration, tissue regeneration, and remodelling. Following tissue destruction, wound healing occurs, thus we reasoned that antibodies might bind to injured tissues, facilitating the engulfment of damaged tissues by macrophages. Here, we demonstrate how B cells participate in wound healing by secreting antibodies against injured tissues. Transfer of spleen cells into splenectomized mice reversed the delay in wound healing caused by splenectomy. Additionally, splenectomized nude mice showed a delay in wound healing. Magnetic beads used to transfer enhanced B220+ cells sped up the healing process in mice with splenectomies. Magnetic beads used to transfer enhanced B220+ cells sped up the healing process in mice with splenectomies. Using anti-IgG1 that has been fluorescein isothiocyanate-labeled, we were able to identify immunoglobulin G1 (IgG1) binding to injured tissues 6–24 hours after the injury. The amount of IgG1 binding to injured tissues was decreased after splenectomy. Studies using immunoblotting showed multiple bands, which were diminished following splenectomy. We discovered that the strength of numerous bands was lower in the serum from splenectomized animals than in that from mice who underwent a sham operation using immunoprecipitation with anti-IgG linked to protein G.

Inflammatory markers are considered as a common biochemical manifestation among subjects with atopic disorders. The current study was aimed to study the possible association of IL-13 and STAT 6 genes among atopic subjects.

A total of 528 patients diagnosed with atopy and 610 normal subjects were taken for the polymorphic analysis of IL-13 and STAT 6 genes. On stratification, we observed a significant difference between cases and controls with various demographic, life style and dietary factors. Subjects harboring homozygous variant genotypes of IL-13–C1055 T and IL-13–A1512 C showed a statistically significant increase in atopy (OR=1.8 (1.2–2.6), P=0.001) and (OR=6.7 (4.9-9.2), P<0.0001) respectively. Similarly, the variant genotype of STAT 6 G2964 A showed a substantial increase towards atopy when harbor by the subjects (OR=3.7 (2.7–5.1), P<0.0001). Additionally, the above genetic variants of all the genes significantly modified the risk of allergy when stratification was done on the basis of various lifestyle, epidemiological and clinico-pathological characteristics.

Helicobacter pylorus is one of the most important human pathogens, infecting more than 50% of the human population. Commonly the first noninvasively testes used for H. pylori infection's detection were immunological tests like blood antibody test and stool antigen test. We investigated the more efficient susceptibility immunological test for detection of H.pylori infection in adult Egyptian patients by comparing the results of H. pylori IgA (HpIgA), IgG in serum blood (HpIgG) and H.pylori Ag in stool (HpSAg) for 30 adult patient (males and females) against control group using ELISA technique.The results showed that each test could be used successfully for diagnosis of H. pylori with 100% specificity and PPV% with no gender relation. Finally HpSAg showed reliable results with high sensitivity (>95%) followed by HpIgG (87.5%), while HpIgA showed the lowest sensitivity (37.5%). Our findings confirms the use of the mentioned immunological tests for detectingthe H. pylori infection and suggest the use H. pylori Ag in stool as the most economic, sensitive and reliable method alone or followed by IgG antibody test as confirmatory test to be the first choices for early diagnosis of H. pylori especially in developing countries.