Journal de génétique et génomes

HPAIV H5N1 was circulated in Egypt since 2006 with recent introduction of H5N8 virus at the end of 2016. AIV has special virus Tropism and infectivity in different isolation host. There for we study the growth kinetics for 9 H5N1 viruses and one H5N8 virus in 3 different tissue culture systems (MDCK, CEF and VERO) and detection the potential bounding to mammalian host. Growth kinetics of cell culture growing viruses was estimated by different parameter as TCID50, CPE percent and HA activity of viruses. Growth kinetics level of Clade 2.2.1.2 and new isolated H5N8 is the highest with early detection of the virus in cell within 1st 24 hr in comparison to another 2 clades of H5N1 viruses. MDCK support high growth kinetics for all clades of virus between 3 types of cell. Antigenic characterization of the viruses supported the results of the phylogenetic analysis. Multiple peculiar mutations were characterized in the Egyptian H5N1 and H5N8 viruses. The Egyptian AI viruses preferentially bound to avian-like receptors rather than human-like receptors. MDCK cell line was suitable isolation host to replace egg isolation system with antigenic and genetic stability. This study describing the growth kinetics of HPAIV H5N1 and H5N8 in various cell lines is an informative study to understand influenza virus establishment and evolution in regard to preventive measures and cure.

Legume lectins are a large group of proteins with similar structures, considerable amino acid sequence homology, and a variety of carbohydrate-binding specificities. Over 363 structures of plant lectins have been characterized, and they are the most extensively studied proteins of this class. In this study, in silico analyses were performed on legume lectin DNA and protein sequences of 35 species, aiming to find their conserved domains and to predict and compare their different tertiary structures, functions and molecular interactions. The protein sequences were aligned with ClustalW algorithm implemented in MEGA 6.06 software and a phylogenetic tree was constructed using the Neighborjoining method. Functional domain analysis revealed eight functional domains present in the four selected sequences representative of the main phylogenetic groups. The identified domains are related to carbohydrate binding, cytokinin binding and protein serine/threonine kinase activities. A model of tertiary structure of Vigna unguiculata was generated by Phyre2 server with the multitemplate feature, and its quality was verified by Molprobity and ProSA-web servers. Results of the molecular docking analysis revealed interaction sites with monosaccharides and a cytokinin from two different binding pockets. These results may provide theoretical informations into the molecular basis of legume lectin functions and structure.

Background: Heteromorphic variants including Yq12 material, being inserted or added to autosomal chromosomes have been reported for chromosomes 1, 7, 11, 13, 14, 15, 21 and 22. Here we describe a novel insertion of Yq12 heterochromatin into a chromosome 17; to the best of our knowledge no similar cases have been reported previously.

Methods: GTG-, C-banding, fluorescence in situ hybridization (FISH), and homemade human heterochromatin specific multicolor FISH probes set (HCM-mix) were used to define the abnormality. A whole chromosome painting (wcp) probe for #17 together with a probe for Yq12 heterochromatin was hybridized to the patient sample. Additionally, Y microdeletion PCR was done to detect possible AZF subregional deletions.

Results: The male patient had normal sperm analysis and no AZF deletions on Y chromosome. GTG and Cbanding showed an additional band on chromosome 17q21. FISH studies revealed that the insertion was derived from Yq12 heterochromatin.

Conclusions: The heterochromatin insertion on 17q21 originating from Yq12 chromosome did not affect the spermatogenesis of aberration carrier and is probably not the cause of infertility in these partners. However, a new heteromorphic variant was identified in this case.