Toxicologie environnementale et analytique

The purpose of the study was to investigate the effects of the secondary biological purification products on the cellular and genotoxic level in the digestive gland and the haemolumph of mussels Mytillus galloprovincialis. The mussels were divided into 3 groups, where the effect of 0.02, 0.2 and 0.5% (v/v) of the biological purification sample was performed respectively after chlorination for 10 and 20 days, while the 4th group was the control animals. The results of the study showed statistically significantly lower NRR times and remarkable sensitivity of the haemocytes to the formation of single-stranded DNA fragments in the mussels exposed at both biological sample purification samples compared to the control ones. Finally, the results of the biomarker "stress assessment" showed statistically significantly less survival time in the exposed mussels compared to controls. Notable is the presence of significant correlations between the values of the biomarkers applied.

Background: Gasoline workers are continuously exposed to many kinds of harmful fumes at their working places. These fumes contain terribly dangerous pollutants which can seriously alter the urinary and hematological profiles. This could be pathological in many conditions.

Aim and Objective: The aim of this study was to determine the effects (if any) of the exposure of gasoline station workers to the gasoline with special focus on CBC parameters and urinary pattern.

Results: In hematological examinations of group-I, WBC Counts, EO, BA, and platelets with indices PCT and MPV were significantly increased (p<0.05). In Group-II, WBC counts, LY, EO, BA and platelets with indices PCT and MPV were significantly increased, whereas RBCs and HGB with their indices HCT, MCV and were significantly decreased. Significant changes in specific gravity, PH-values, Nitrate, Protein, Ketone, UGB and Bilirubin were also investigated. Significant weak negative correlations were found among RBC (r=-0.3, p=0.003), HGB (r=-0.3, p=0.003), HCT (r=-0.3, p=0.003) and MCV (r=-0.22, p=0.03) with the exposure length. Significant weak positive correlation of MO (r=0.23, p=0.03), BA (r=0.22, p=0.04) were observed with the exposure length in gasoline filling workers.

Conclusion: The present study concluded that prolong exposure of gasoline fumes cause massive alteration in hematological parameters particularly WBC counts with differentials LY, EO, BA, RBC and HGB with their indices HCT, MCV, PLT, PCT and MPV as well as Nitrate, UGB and Bilirubin in urine. These alterations in hematology and urine composition due to toxicities of gasoline fume produce high levels of many serious abnormalities and illness in human health like bone marrow depression, immune depressing and lymphatic leukemia.