Métabolomique : accès libre

Urinary Metabolic Profiling of MARS-500 Project with Nuclear Magnetic Resonance Spectroscopy

Abstract

Chen P, Yu Y, Dong H, Li H, Huang J, Zang J, Lv K, Zhao G and Chen B

Human sustainable space exploration could go further with the development of strategies and preparatory activities. Enormous scientific work has been done during the 21st century. MARS-500 project is one of unique preparatory activities, and was finished within 520 day isolation on the Earth. The full 520 day MARS mission simulation provided an opportunity to conduct a long-term, isolated experiment to observe metabolic changes due to people differences and its stability to duration variation. Six volunteers were selected from Russia, France, Italy, and China, and their urine specimens were collected in six periods. Then the subtle metabolic variations were investigated by nuclear magnetic resonance (NMR) at six different periods. We found that the metabotypes of six crews drifted out from the first period to the farthest third period, then reverted to the nearest sixth period in metabolic space, suggesting a property of resilience for metabolic phenotype. Furthermore, a set of 20 biomarkers, discriminating great changes in metabolic phenotypes well, were associated with emotion, muscle turnover, circadian rhythms, energy expenditure, lipid metabolism, oxidative activity, cognitive performance, and gut-microbial activities. In deeps, to analysis the data from MARS-500 project with two-way ANOVA, an interaction between factor of people differences and long-term isolation showed that the six crews each had a specific response to 520 days’ isolation. In this picture, accurate healthy management during future human space mission should aim at promoting his or her ability to
revert to his or her stable metabotype under a defined and isolated environment.