Science et technologie vétérinaires

The Use of Real-Time PCR (qPCR) for the Diagnosis of Bovine Herpes virus 5 in Formalin-Fixed, Paraffin-Embedded Bovine Brain Samples Identified as Bovine Unspecific Encephalitis

Abstract

Francisco Pedraza-Ordoñez, Ricardo Seiti Yamatogi, Joao Pessoa Araujo Jr., Noeme Sousa Rocha and Antonio Carlos Alessi

Background: Bovine herpes virus 5 (BoHV-5) is a neurovirulent alpha herpes virus that causes fatal meningoencephalitis and huge economic losses in the livestock and associated industries worldwide; its diagnosis in the laboratory is essential for disease control, immunologic surveillance and effective monitoring programs in endemic areas.

Materials and Methods: In this study, brain samples from 21 Colombian cattle that died of neurological disease, were negative for rabies and were diagnosed with nonspecific encephalitis were processed by real-time PCR (qPCR) in the laboratory of virology at the Institute of Biosciences, Sao Paulo State University, Brazil, to detect BoHV-5 genetic material in tissue preserved in paraffin blocks. Tissues were considered positive for BoHV-5 if ampliï¬Âcation occurred at or before 30 PCR cycles, inconclusive if amplification occurred between 30 and 40 cycles and negative otherwise.

Results and Conclusion: Two cases were positive, demonstrating that qPCR may be useful for confirming herpes encephalitis in acid formalin-fixed tissues with an inconclusive diagnosis.