Toxicologie environnementale et analytique

Protective Effect of a Synthetic Antioxidant "Acetyl Gallate Derivative" Against Dimethoate Induced DNA Damage and Oxidant/Antioxidant Status in Male Rats

Abstract

Tarek M. Heikal*,Abdel-Tawab H. Mossa,Galal A. M. Nawwar,Mahmoud El-Sherbiny,Hassan Z. Ghanem

The present study was conducted to investigate the protective effects of a synthetic antioxidant “acetyl gallate derivative” (SAC) against hepatic oxidative stress and brain DNA damage induced by dimethoate (DM) in male rats. DM was orally administrated to the rats at a dose of 38.7 mg kg-1 b.wt. (1/10 LD50), for 28 consecutive days. Additional DM groups received either SAC or vitamin C (VC) at a dose of 200 mg kg-1 b.wt. 30 min before DM administration. Compared to the control, DM induced a statistical reduction in body weight gain, while induced a statistical increase in absolute and relative liver weights. Oral administration of DM significantly caused increases in hepatic lipid peroxidation (LPO) and activities of antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD) and glutathione-s-transferease (GST), while caused decreases in glutathione content (GSH) and serum cholinesterase(ChE) activity. Administration of SAC attenuates LPO, GSH content and antioxidant enzymes system. The severity of brain DNA damage monitored by damage index (DI) and damage frequency % (DF) induced by DM was mitigated after administration of SAC. In conclusion, supplementation of SAC is more reliable than VC in attenuating relative liver weight, SOD, GST, and brain DNA damage.