Journal d'histologie moléculaire et de physiologie médicale

New Technique to Diagnose Tissue microarray

Abstract

Darshini Arpi

A recent development in pathology is tissue microarray. A microarray enables high throughput analysis of multiple specimens at once because it contains numerous small representative tissue samples from hundreds of distinct instances combined on a single histologic slide. Tissue microarrays are paraffin blocks created by re-embedding cylindrical tissue cores at predetermined array coordinates into a single recipient (microarray) block from various paraffin donor blocks. This method enables the arraying of up to 1000 tissue samples or more into a single paraffin block. On a single glass slide, it can allow simultaneous study of molecular targets at the DNA, mRNA, and protein levels under the same, uniform circumstances, as well as maximise the preservation and utilisation of finite and priceless archival tissue samples. This adaptable method, which automates data processing, makes it easier to conduct both retrospective and prospective human tissue investigations. It has a variety of potential uses in fundamental research, prognostic oncology, and drug discovery. It is a useful and efficient technique for high-throughput molecular analysis of tissues that is assisting in the identification of new diagnostic and prognostic markers and targets in human cancers. The pros, uses, and restrictions of tissue microarray fabrication and sectioning are outlined in this article.