Journal des biomarqueurs moléculaires et du diagnostic

In vitro Evaluation of Cobalt-Zinc Ferrite Nanoparticles Coated with DMSA on Human Prostate Cancer Cells

Abstract

Daryoush Shahbazi-Gahrouei, Zeinab Ghasemian, Mohammad Abdolahi, Sohrab Manouchehri, Shaghayegh haghjooy Javanmard and Nasim Dana

Toxicity of nanoparticles on the human health is one main feature for successful application of nanoparticles in medicine. In this paper, the cytotoxicity effects of CZF-MNPs and CZF-MNPs @ DMSA were evaluated. For this purpose, at first the characterization of Cobalt-Zinc Ferrite Magnetic Nanoparticles (CZF-MNPs) coated with biocompatible Dimercaptosuccinic Acid (DMSA) was investigated using of Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FTIR) and Atomic Absorption Spectrophotometer (AAS). In following, the cytotoxicity effects of CZF-MNPs and CZF-MNPs @ DMSA were investigated on human prostate cancer cell lines, HPCs, (PC3 and DU145). The results showed that the average size of bare and coated nanoparticles was about 16 and 40 nm. The FTIR spectra results showed the presence of DMSA cover on the surface of nanoparticles. Furthermore, in vitro MTT assay CZF-MNPs @ DMSA at high concentrations (1.2 and 1.5 mM Fe) study results showed that they have some cytotoxicity on HPCs (PC3 and DU145).