Journal de médecine légale

Genetic Variation for Nine Autosomal Short Tandem Repeat (STRs) in Egyptian Population

Abstract

Tarek Tahaa, Sagy Elzalabanyb, Olfat Shaker and Sahar Fawz

Allele frequencies for 9 autosomal short tandem repeat loci (STRs) D3S1358, VWA, FGA, THO1, TPOX, CSF1PO, D5S818, D13S317, D7S820 and a locus allowing for sex discrimination were determined in 1000 unrelated Egyptians. Blood samples were collected after obtaining an informed consent. The DNA was extracted by using the standard phenol-chloroform extraction method and then amplified using commercial available kit (AmpF L STR profiler kit, Applied Bio systems) by the polymerase chain reaction, and subsequently typed using capillary electrophoresis (3130X1 Genetic Analyser, Applied Bio systems). All loci met Hardy-Weinberg expectations and no mutations were found among subjects.