Toxicologie environnementale et analytique

Fluoride Induces Morphological and Biochemical Changes in Goat Eye Lens

Abstract

Sudhanshu Mishra, Swati Tomar, Anant Sharma, Dushyant Singh Chauhan and Sandeep Tripathi

In the present study, we attempted to find out the biochemical changes in goat eye lens due to Fluoride. Twenty four goat eye lenses were divided into four groups (N=6). Three experimental groups were incubated with three different concentration of Fluoride (50, 100 and 200 ppm) for 24 hours at room temperature. After incubation, lens were homogenised to estimate Lipid peroxide level (LPO), Protein carbonyl content (PC), total protein (TP), GSH and antioxidant enzymes such as SOD and CAT. We observed that gradually, LPO and PC were significantly (p<0.001) increased with the fluoride concentration while reduced glutathione level (GSH) and antioxidant enzymes, superoxidedismutase (SOD) and catalase (CAT) were significantly decreased with the increased concentration of fluoride. On the basis of the results, It may conclude that uptake of excess consumption of Fluoride may be linked with increased oxidative burden which may lead to lens opacification, progression and sometimes leads to development of cataract.