Toxicologie environnementale et analytique

Comet Assay on Toxicogenetics; Several Studies in Recent Years on Several Genotoxicological Agents

Abstract

Tarek Gharsalli

Cancer is one of the main causes of death in the world. Prolonged exposure to genotoxic chemicals observed is one of the primary causes of cancer. A number of assays exist for detection of genotoxicity in a variety of experimental systems. The Comet assay also known as the single cell gel electrophoresis assay is used to detect DNA damage as an indicator of exposure to geno toxicogical agents. The Comet assay is a broadly used method in human, environmental, and eco genotoxicological studies. The aim of our review is to describe Comet Assay protocol with the advantages and limits and to develop some recent studies in which the authors have used comet test to confirm or reverse agent’s genotoxicity.