Archives d'oncologie chirurgicale

Can piRNAs change the fate of breast cancer cells?

Abstract

Cagri ONER

piR-651 is a member of small non-coding RNAs which is called PIWI Interacting RNAs. piRNAs can regulate the function of transposons by both epigenetic and posttranscriptional silencing. Several studies indicated that piR-651 might be useful for diagnostic of cancer cases according to its oncogenic characteristics. In this study, we aimed to determine the effect of piR-651 on the molecular characteristics of MCF-7 breast cancer cells. Anti-piR-651 sequence was transfected to MCF-7 cells and then the effect of piR-651 on proliferation, adhesion and motility characteristics of cells were detected by using XTT kit procedure at 24th, 48th and 72nd hours. After determining the cellular behaviour changes, to determine the cellular changes of proliferation Ki-67; adhesion MMP-2 and MMP-9 gene expressions were determined by using RT-PCR. According to obtained data, inhibition of piR-651 caused to a decrease on proliferation and adhesion of MCF-7 cells (p<0.001). Ki-67 and MMP- 9 gene expressions are also supported the cellular behaviour assays of piR-651 inhibited MCF-7 cells (p<0.001). Moreover, motility assay showed that the migration rate of piR-651 inhibited MCF-7 cells were more Notes: slowly compared to untreated and non-target treated cells. In benign breast cancer cells line, piR-651 inhibition can change both cellular characteristics and gene expressions which were related to these characteristics. Especially, adhesion and proliferation are the important cellular marks of MCF-7 cells. piR-651 inhibition cause to decrease both proliferation and adhesion of cells, these results have shed on light whether we can mitigate the effects of cancer cells on the organism with piRNA inhibition. Our results indicated that changing the expression patterns of piRNAs might cause to change the fate of benign breast cells.