Journal de la biodiversité et des espèces menacées

Bacterial Populations are Affected by DNA Extraction and Sampling Techniques

Abstract

Chengwei Li*

The amount and quality of DNA produced, as well as its appropriateness for subsequent PCR amplifications, varied significantly depending on the DNA extraction technique used. Quantitative PCR enumeration of microbial marker loci was used to further evaluate DNA extracts from nine extraction techniques that satisfied these initial quality standards. Depending on the DNA extraction technique utilised, the same rumen samples' absolute bacteria counts varied by more than 100-fold. Using 454 Titanium pyro sequencing, the apparent compositions of the archaeal, bacterial, ciliate protozoal, and fungal communities in similar rumen samples were evaluated.