Journal de médecine légale

Allelic Frequencies and Statistical Parameters Determination, for 16 Autosomal STR Markers Included in the NGM Detect^TM PCR Amplification Kit, for the Bulgarian Population

Abstract

Plamen Iliev, Vera Djeliova, Ekaterina Angelova, Bogdan Mirchev, Milka Mileva, Atanas Hristov, Dimo Krastev and Aleksandar Apostolov

In the presented study, the allelic frequencies and statistical parameters for 500 unrelated individuals from the 28 administrative regions of the Republic of Bulgaria were determined for 16 autosomal genetic markers (STR), included in the NGM Detect™ PCR Amplification Kit (Applied Biosystems), for the Bulgarian population. No deviations from Hardy-Weinberg equilibrium for the 16 studied loci were observed, except for a deviation for the D8S1179 and FGA loci. The most polymorphic and informative for the Bulgarian population is the SE marker, with the next most informative markers being: D1S1656, D12S391 and D18S51. For all 16 loci, the combined value Match Probability (MP) is 1.20e-21 and the power of exclusion (PE) is 99.999999022%.

The results obtained from the DNA analysis have a very high identification value. Their interpretation is particularly important when a DNA profile found at a crime scene matches that of a suspect or an injured person. Such statistical estimates are also valuable in detecting mixed DNA profiles, as well as in calculating the probability of kinship between two or more individuals. For these calculations, it is necessary to perform a statistical analysis of the occurrence frequency of the specified STR variants among a given population group of individuals. So far, no such research representative of the Bulgarian population has been published.