Journal de recherche médico-légale

Allele Frequency of 15 Short Tandem Repeats (Strs) in a Buganda Population (Central Uganda): Forensic Utility and Parentage Testing

Abstract

Jane Nabwowe, Musa Kirya, Emmanuel Okello and Ann Nanteza

The allele frequency of 15 Short tandem repeat (STRs) loci of the Buganda population-Central Uganda was determined in 221 unrelated individuals from the randomly selected 10 districts of the Buganda region. The DNA samples were extracted following Chelex extraction protocol, amplified using the AmpFlSTR® Identifiler Plus™ PCR amplification kit, and separated using capillary electrophoresis on an ABI 3130xl Genetic Analyzer. All loci were in Hardy-Weinberg equilibrium expectations and the Buganda population is heterozygous. When combined, combined power of exclusion (CPE), combined match probability (CMP) and combined power of discrimination (CPD) were 0.99981, 1 in 2.471×1017 and 0.999999998 respectively an indication that the loci are highly informative, polymorphic and discriminative useful in paternity and forensic testing in the Buganda population.