Toxicologie environnementale et analytique

Adaptation of the Micronucleus Technique in Allium Cepa, For Mutagenicity Analysis of the Jamari River Valley, Western Amazon, Brazil

Abstract

Dionatas Ulises de Oliveira Meneguetti*,Francisco Carlos da Silva,Renato André Zan,Leandro José Ramos

This study aimed to make technique adjustment of micronuclei in Allium cepa, recommended by Fiskejö [1], by replacing methanol and Giemsa by triarilmetano the 0,1%, xantenos the 0,1% and tiazinas the 0,1%. We obtained good results with the replacement, as its efficiency in stain nucleic acids, obtaining a good visualization of cells, mitotic index, micronuclei, anaphase and telophase bridges, and this methodology is indicated in future examinations of mutagenicity in the river Vale do Jamari.